Laboratory sessions will be available for a limited number of participants.

Participants can select between the 3 following topics:

Topic A - Non-invasive ultrasound imaging

The course will comprise a hands-on laboratory session on non-invasive ultrasound imaging for mouse and rat analysis of soft tissues and cardiac performance. The participants will be able to perform ultrasound imaging and monitor key physiological parameters, such as heart rhythm, using the high-frequency/high-resolution Vevo2100 digital imaging platform.

 

Topic B - Imaging flow cytometry for quantification of cellular parameters.

Imaging Flow Cytometry is a technology that combines the advantages of flow cytometry with those of microscopy, enabling fast acquisition of images (both bright field and fluorescence) of each single cell, and the qualitative and quantitative analysis of several parameters extracted from such images. In this lab session, the participants will have the opportunity to run labelled cells on an Imaging Flow Cytometer (ImageStreamX, from Amnis) and analyse them to quantify parameters, such as: cell morphology, marker(s) internalization, nuclear translocation, cell conjugation, amongst others.

 

Topic C - Confocal Raman Microscopy

Confocal Raman microscopy probes the interaction of monochromatic laser light with chemical bonds, providing information rich spectra, giving detailed insight into the chemical composition of the sample. In this laboratory session the participants will have the opportunity to identify molecular constituents in cells and to obtain Raman mapped images of their distribution.